CARDIOPROTECTIVE EFFECTS OF PHARMOCOLOGICAL POSTCONDITIONING IN THE EXPERIMENTAL MODEL OF ISOLATED RAT HEART REPERFUSED AFTER COLD CRYSTALLOID CARDIOPLEGIA
Keywords:
isolated heart, cardioplegic ischemia, reperfusion, levosimendan, postconditioningAbstract
Aim: To assess contractile function and the degree of myocardial damage during hypothermic ischaemia and reperfusion in isolated rat hearts, postconditioned with levosimendan. Material and methods. To estimate the extent of myocardial damage, the values of the markers for myocardial necrosis, namely CK MB, LDH, were measured. To assess the myocardial contractile function, coronary blood flow, heart rate, left ventricular pressure were evaluated. The dynamics of the redox processes during reperfusion have been studied. The histological study was performed. Results. Cardioprotective effects of levosimendan in isolated heart reperfused after cardioplegic ischemia have been confirmed. The overall effect results in a reduction of reperfusion induced release of enzyme markers for myocardial damage, a reduction of the severity of myocardial pathological changes and a reduction of the intensity of free radical reactions in the myocardium. Conclusion. Reduced severity of cardiomyocytes damage and restoration of myocardial contractile function using the cardioprotective drug levosimendan resulted in the reduction of intensification of free radical oxidation in isolated rat heart model.
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